Vibrio natriegens transformations

So far I have gotten vibrio natriegens transformants using electroporation with pRL1342, which is addgene Plasmid #70691. They grow into large colonies overnight at room temperature.

Chloramphenicol stocks have to be diluted approx. 1/25 from E coli usage because V. nat is very sensitive to that condition. I had originally tried room temperature creation of electrocompetent cells according to “Vibrio natriegens, a new genomic powerhouse”, and not gotten transformants due to this. I will be attempting this protocol again at room temperature.

I got decent transformation efficiency, but nowhere near what I need for actual cloning. Will be investigating possible knockouts to increase efficiency. (50 colonies with 50ng plasmid approx)

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