Quick Yeast gDNA prep

MATERIALS

  • 70% and 100% Ethanol
  • 200mM LiAc, 1% SDS solution

PROTOCOL

  1. Pick one yeast colony from the plate or spin down 100-200 μl of liquid yeast culture (OD600=0.4). Suspend cells in 100 μl of 200mM LiOAc, 1 % SDS solution.
  2. Incubate for 5 minutes at 70°C.
  3. Add 300μl of 96-100 % ethanol, vortex.
  4. Spin down DNA and cell debris at 15 000 g for 3 minutes.
  5. Wash pellet with 70 % ethanol
    • Spin down and pipet out excess ethanol
    • Dry pellet
  6. Dissolve pellet in 100 μl of H2O or TE and spin down cell debris for 15 seconds at 15000g.
  7. Use 1 μl of supernatant for PCR.

from: EXTRACTION OF GENOMIC DNA FROM YEASTS FOR PCRBASED APPLICATIONS, Biotechniques 2011