I am cloning F1 origin into pKGs2, which will then be used as a template for my other reactions on this ‘base’ vector. Should have it done very soon. Will update upon completion.
In other news, I bought a MacConnell MP4800 for automated minipreps. I am going to do a full write up on the machine later. Also bought a gene pulser electroporator. I am going to 3d print the safety part for the shock chamber, will post file when that is complete.