KG_16 | B.subtilis CAL306, 1A1121


(PMID: 22505685) Strain contains ICEBs1 under induction of Pspank promoter. ICEBs1 selected with kanamycin, Pspank-rapI integrated at amyE locus selected with spectinomycin. Can actively conjugate rolling-cycle plasmids.


Lee CA, Grossman AD. Identification of the origin of transfer (oriT) and DNA relaxase required for conjugation of the integrative and conjugative element ICEBs1 of Bacillus subtilis. J Bacteriol. 2007 Oct;189(20):7254-61 (PMID: 17693500)



Location Designation Quick Name Other Other-2
16 KG_16 B.subtilis CAL306, 1A1121 ΔnicK306, Δ(rapI-phrI)342::kan, amyE::{(Pspank(hy)-rapI) spc} 37. Kan. Spc.

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