Very high levels of competence induced by xylose (%, w/v); derived from strain 1A751; ganA was formerly known as lacA; if there are two colony sizes on a plate of this strain, choose the smaller for competence induction.
Zhang XZ, Zhang YH. Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis. Microb Biotechnol. 2011 Jan;4(1):98-105 (PMID: 21255377)
|13||KG_13||B.subtilis SCK6, 1A976||eglSΔ102, bglT/bglSΔEV, nprE18, aprEΔ3, his ganA::PxylA-comK Em||37. Erm.|
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